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KMID : 0859320100280030141
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Journal of the Korean Society for Therapeutic Radiology and Oncology
2010 Volume.28 No. 3 p.141 ~ p.146
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Protective Effects of 5-Androstendiol (5-AED) on Radiation-induced Intestinal Injury
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Kim Joong-Sun
Lee Seung-Sook
Jang Won-Suk
Lee Sun-Joo
Park Sun-Hoo
Cho Soo-Youn
Moon Chang-Jong
Kim Seong-Ho
Kim Mi-Sook
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Abstract
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Purpose:We examined the radioprotective effects of 5-androstendiol (5-AED), a natural hormone produced in
the reticularis of the adrenal cortex, as a result of intestinal damage in gamma-irradiated C3H/HeN mice.
Methods: Thirty mice (C3H/HeN) were divided into three groups; 1) non-irradiated control group,
2) irradiated group, and 3) 5-AED-treated group prior to irradiation. Next, 5-AED (50 mg/kg per body weight)
was subcutaneously injected 24 hours before irradiation. The mice were whole-body irradiated with 10 Gy for the
histological examination of jejunal crypt survival and the determination of the villus morphology including crypt
depth, crypt size, number of villi, villus height, and length of basal lamina, as well as 5 Gy for the detection of
apoptosis.
Results:The 5-AED pre-treated group significantly increased the survival of the jejunal crypt, compared to
irradiation controls (p＜0.05 vs. irradiation controls at 3.5 days after 10 Gy). The evaluation of morphological
changes revealed that the administration of 5-AED reduced the radiation-induced intestinal damages such as
villus shortening and increased length of the basal lamina of enterocytes (p＜0.05 vs irradiation controls on 3.5
day after 10 Gy, respectively). The administration of 5-AED decreased the radiation-induced apoptosis in the
intestinal crypt, with no significant difference between the vehicle and 5-AED at 12 hours after 5 Gy.
Conclusions:The results of this study suggest that the administration of 5-AED has a protective effect on
intestinal damage induced by γ-irradiation. In turn, these results suggest that 5-AED could be a useful
candidate for radioprotection against intestinal mucosal injury following irradiation.
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KEYWORD
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5-Androstenediol, Radiation, Intestinal crypt, Intestinal injury
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